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Miltenyi Biotec magnetic activated cell sorting
(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
Magnetic Activated Cell Sorting, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Miltenyi Biotec magnetic cell sorting system
(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
Magnetic Cell Sorting System, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
Macs Magnetic Labeling Based Cell Sorting Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
Magnetic Activated Cell Sorting Macs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
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(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
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Miltenyi Biotec anti myelin magnetic bead sorting
(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
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Miltenyi Biotec magnetic activated cell sorting columns
(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
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Image Search Results


(A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; MACS, magnetic-activated cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .

Journal: PLOS One

Article Title: Significance of RGS13 expression in lupus B cells

doi: 10.1371/journal.pone.0348945

Figure Lengend Snippet: (A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; MACS, magnetic-activated cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .

Article Snippet: B cells were further separated from the peripheral blood mononuclear cells via magnetic-activated cell sorting (MACS; B Cell Isolation Kit II human; Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Cell Culture, Knock-Out, FACS